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Laboratory for Metabolism and Bioenergetics focusses on the research into mitochondrial function and dysfunction in human health and disease. Our projects deal with the availability of nutrients, gluco- and lipotoxicity and their relation to the metabolic syndrome and type 2 diabetes, with oxidative stress and mtDNA damage, skeletal muscle and cancer cell metabolism and the impact of drugs and environmental factors on mitochondrial function.

We were the first laboratory in the Czech Republic to start using the Seahorse metabolic flux analyzer, thanks to which we have unparalled expertise and experience in the fieed of metabolic analysis. Our lab also makes use of standard molecular biological methos, confocal and electron microscopy, a range of bioenergetic methods, metabolomics and enzymological and biochemical analyses. We collaborate on projects with our colleagues in the Královské Vinohrady hospital and other institutions.

For the academic year 2020/21 we offer two PhD positions in cancer metabolism (deadline 30th April 2020)

Group leader: doc. MUDr. Jan Trnka, Ph.D., M.Phil., M.Sc. ORCID

Assistant Professor: ing. Stanislava Martínková, Ph.D.

Post-doc: MUDr. Adéla Krajčová. Ph.D.

Ph.D. students:

Mgr. Anežka Kafková

Alumnae/i:

Mgr. Jana Tůmová (Patková), Ph.D. (2009-2016)

Moustafa Elkalaf, MBBCh, Ph.D. (2009-2019)

Our recent publications:

Moráň Lukáš; Pivetta Tiziana; Masuri Sebastiano; Vašíčková Kateřina; Walter Franziska; Prehn Jochen; Elkalaf Moustafa; Trnka Jan; Havel Josef; Vaňhara Petr. Mixed copper(ii)-phenanthroline complexes induce cell death of ovarian cancer cells by evoking the unfolded protein response. Metallomics,11,9,1481-1489

Verlande Amandine; Krafčíková Michaela; Potěšil David; Trantírek Lukáš; Zdráhal Zbyněk; Elkalaf Moustafa; Trnka Jan; Souček Karel; Rauch Nora; Rauch Jens; Kolch Walter; Uldrijan Stjepan. Metabolic stress regulates ERK activity by controlling KSR-RAF heterodimerization. EMBO Reports, 19,2,320-336

Krajčová Adéla; Løvsletten Nils G.; Waldauf Petr; Frič Vladimír; Elkalaf Moustafa; Urban Tomáš; Anděl Michal; Trnka Jan; Thoresen G. Hege; Duška František. Effects of Propofol on Cellular Bioenergetics in Human Skeletal Muscle Cells. Critical Care Medicine, 46,3,e206-e212

Gojda Jan; Rossmeislová Lenka; Straková Radka; Tůmová Jana; Elkalaf Moustafa; Jaček Martin; Tůma Petr; Potočková Jana; Krauzová Eva; Waldauf Petr; Trnka Jan; Štich Vladimír; Anděl Michal. Chronic dietary exposure to branched chain amino acids impairs glucose disposal in vegans but not in omnivores. European Journal of Clinical Nutrition,71,5,594-601

Elkalaf, M.; Weiszenstein, M.; Polák, J.; Trnka, Jan: Mitochondrial Probe Methyltriphenylphosphonium (TPMP) Inhibits the Krebs Cycle Enzyme 2-Oxoglutarate Dehydrogenase. PLoS One, 2016, 11(8): e0161413. DOI: 10.1371/journal.pone.0161413.

Jiroutková, K.; Krajčová, A.; Žiak, J.; Fric, M.; Gojda, J.; Džupa, V.; Kalous, M.; Tůmová, J.; Trnka, Jan; Duška, F.: Mitochondrial Function in an In Vitro Model of Skeletal Muscle of Patients With Protracted Critical Illness and Intensive Care Unit-Acquired Weakness. J. Parenter. Enter. Nutr., 2016, Online June 29, 2016(v tisku): DOI: 10.1177/0148607116657649.

Tůmová, J.; Anděl, M.; Trnka, Jan: Excess of free fatty acids as a cause of metabolic dysfunction in skeletal muscle. Physiol. Res., 2016, 65(2): 193-207.

Weiszenstein, M.; Pavlíková, N.; Elkalaf, M.; Halada, P.; Šeda, O.; Trnka, Jan; Kovář, J.; Polák, J.: The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware. PLoS One, 2016, 11(3): e0152382. DOI: 10.1371/journal.pone.0152382.

Weiszenstein, M.; Mušutová, M.; Plíhalová, A.; Westlake, K.; Elkalaf, M.; Koc, M.; Procházka, A.; Pala, J.; Gulati, S.; Trnka, Jan; Polák, J.: Adipogenesis, lipogenesis and lipolysis is stimulated by mild but not severe hypoxia in 3T3-L1 cells. Biochem. Biophys. Res. Commun., 2016, 478(2): 727-732. DOI: 10.1016/j.bbrc.2016.08.015.

Dudák, J.; Žemlička, J.; Krejčí, F.; Polanský, Š.; Jakubek, J.; Mrzílková, J.; Patzelt, M.; Trnka, Jan: X-ray micro-CT scanner for small animal imaging based on Timepix detector technology. Nucl. Instrum. Methods Phys. Res. A, 2015, 773(February): 81-86. DOI: 10.1016/j.nima.2014.10.076.

Finichiu, P.G.; Larsen, D.S.; Evans, C.; Larsen, L.; Bright, T.P.; Robb, E.L.; Trnka, Jan; Prime, T.A.; James, A.M.; Smith, R.A.; Murphy, M.P.: A mitochondria-targeted derivative of ascorbate: MitoC. Free Radic. Biol. Med., 2015, 89(12): 668-678. DOI: 10.1016/j.freeradbiomed.2015.07.160.

Jiroutková, K.; Krajčová, A.; Žiak, J.; Fric, M.; Waldauf, P.; Džupa, V.; Gojda, J.; Němcová-Fürstová, V.; Kovář, J.; Elkalaf, M.; Trnka, Jan; Duška, F.: Mitochondrial function in skeletal muscle of patients with protracted critical illness and ICU-acquired weakness. Crit. Care, 2015, 19(1): Article 448. DOI: 10.1186/s13054-015-1160-x.

Krajčová, A.; Žiak, J.; Jiroutková, K.; Patková, J.; Elkalaf, M.; Džupa, V.; Trnka, Jan; Duška, F.: Normalizing Glutamine Concentration Causes Mitochondrial Uncoupling in an In Vitro Model of Human Skeletal Muscle. J. Parenter. Enter. Nutr., 2015, 39(2): 180-189. DOI: 10.1177/0148607113513801.

Pavlíková, N.; Weiszenstein, M.; Pala, J.; Halada, P.; Šeda, O.; Elkalaf, M.; Trnka, Jan; Kovář, J.; Polák, J.: The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes. Cell. Mol. Biol. Lett., 2015, 20(5): 919-936. DOI: 10.1515/cmble-2015-0054.

Trnka, Jan; Elkalaf, M.; Anděl, M.: Lipophilic triphenylphosphonium cations inhibit mitochondrial electron transport chain and induce mitochondrial proton leak. PLoS One, 2015, 10(4): e0121837. DOI: 10.1371/journal.pone.0121837.

Tůmová, J.; Mališová, L.; Anděl, M.; Trnka, Jan: Protective Effect of Unsaturated Fatty Acids on Palmitic Acid-Induced Toxicity in Skeletal Muscle Cells is not Mediated by PPAR delta Activation. Lipids, 2015, 50(10): 955-964. DOI: 10.1007/s11745-015-4058-0.

Patková, J.; Anděl, M.; Trnka, Jan: Palmitate-Induced Cell Death and Mitochondrial Respiratory Dysfunction in Myoblasts are Not Prevented by Mitochondria-Targeted Antioxidants. Cell. Physiol. Biochem., 2014, 33(5): 1439-1451. DOI: 10.1159/000358709.

Elkalaf, M.; Anděl, M.; Trnka, Jan: Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes. PLoS One, 2013, 8(8): e70772. DOI: 10.1371/journal.pone.0070772.

Gojda, J.; Patková, J.; Jaček, M.; Potočková, J.; Trnka, Jan; Kraml, P.; Anděl, M.: Higher insulin sensitivity in vegans is not associated with higher mitochondrial density. Eur. J. Clin. Nutr., 2013, 67(12): 1310-1315. DOI: 10.1038/ejcn.2013.202.